Recombination intermediates

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Resolution of Recombination Intermediates: Mechanisms and Regulation.

DNA strand break repair by homologous recombination leads to the formation of intermediates in which sister chromatids are covalently linked. The efficient processing of these joint molecules, which often contain four-way structures known as Holliday junctions, is necessary for efficient chromosome segregation during mitotic division. Because persistent chromosome bridges pose a threat to genom...

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The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates.

During meiosis, accurate segregation of intact chromosomes is essential for generating healthy gametes. Defects in recombination and/or chromosome synapsis activate the pachytene checkpoint, which delays meiotic cell cycle progression to avoid aberrant chromosome segregation and formation of defective gametes. Here, we characterize the role of the conserved DNA damage checkpoint protein Ddc2/AT...

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Identification of double holliday junctions as intermediates in meiotic recombination

During meiosis, branched DNA molecules containing information from both parental chromosomes occur in vivo at loci where meiosis-specific double-stranded breaks occur. We demonstrate here that these joint molecules are recombination intermediates: they contain single strands that have undergone exchange of information. Moreover, these joint molecules are resolved into both parental and recombin...

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Single Holliday Junctions Are Intermediates of Meiotic Recombination

Crossing-over between homologous chromosomes facilitates their accurate segregation at the first division of meiosis. Current models for crossing-over invoke an intermediate in which homologs are connected by two crossed-strand structures called Holliday junctions. Such double Holliday junctions are a prominent intermediate in Saccharomyces cerevisiae meiosis, where they form preferentially bet...

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Intermediates in serine recombinase-mediated site-specific recombination.

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ژورنال

عنوان ژورنال: Nature

سال: 1981

ISSN: 0028-0836,1476-4687

DOI: 10.1038/289097c0